| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061827 |
| Chromosome: | chromosome 7 |
| Location: | 5880194 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353837 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCGACACTGAGTGCGACACATTGGCCAACGTCCTTGCCAACGCGCCC |
| Internal bar code: | TGTCGACCGGGTCCGTTTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCCCCATGCCACGCTAAA |
| Suggested primer 2: | AGGGTCTGACGCTGTTGAAG |