Insertion junction: CLIP2.061834_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.061834
Chromosome:chromosome 6
Location:334734
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g251100 NMT1 (1 of 1) 2.3.1.97 - Glycylpeptide N-tetradecanoyltransferase / Peptide N-tetradecanoyltransferase; N-myristoyl transferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCCTCCTCAATGCTAGGGCTCACGCCCACGTACAACCCCGATCCCC
Internal bar code:GAGCACAGGTACATACCCTAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1708
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAGGTTCCAGAACATCGA
Suggested primer 2:ATTCCATGCTCACCCAGTGG