| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.061835 |
| Chromosome: | chromosome 13 |
| Location: | 3244215 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585600 | (1 of 1) IPR011333//IPR019832 - POZ domain // Manganese/iron superoxide dismutase, C-terminal | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGACAAGTGAAGCTGATATTACAGCAGAAAACCCAAGCGTCAACCAG |
| Internal bar code: | GCACTTGAATGGGTAGCTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 979 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTCCTTCAGCGTGTTCT |
| Suggested primer 2: | CTAGGACGTGGACATGGACG |