Insertion junction: CLIP2.061836_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061836
Chromosome:chromosome 7
Location:1374993
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g322884 (1 of 5) 2.4.1.17 - Glucuronosyltransferase / Uridine diphosphoglucuronyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGTGATCAAACCCCGAAAACACACGAAGTTGGCGCAAATGCAACGAA
Internal bar code:AGATTGGATAGGAGCGTTGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2036
LEAP-Seq percent confirming:94.2308
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:ATACACACCCTCCGTACCCA
Suggested primer 2:GGCTGTTTGTCGTTTGACCC