| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061837 |
| Chromosome: | chromosome 2 |
| Location: | 865161 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078912 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCCTCAGCTCATGCCGCAAGACAACTAACACTACCGTACGCCGCTGC |
| Internal bar code: | TAAACGAGTGTCGTGTTCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1936 |
| LEAP-Seq percent confirming: | 93.2203 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGAGCTAGTGTTGCGCTT |
| Suggested primer 2: | GGCATCGTCACACGTATTGC |