Insertion junction: CLIP2.061855_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.061855
Chromosome:chromosome 17
Location:1605457
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g707500 TOC120,TOC159 Translocon at the outer envelope membrane of chloroplasts, receptor subunit; (1 of 1) PF11886 - Translocase of chloroplast 159/132, membrane anchor domain (TOC159_MAD) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCGAACCCAATGCAAAAGAGCGAGGTGTGTGTATGTGGGGGGGGGGGG
Internal bar code:CGTGATGGATTTTTCGAGTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:102
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCACACAGTACACAGGAC
Suggested primer 2:CAACCTGGGTTGCATGTGTG