Insertion junction: CLIP2.061881_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.061881
Chromosome:chromosome 1
Location:605598
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g003524 (1 of 1) PF13499//PF14531 - EF-hand domain pair (EF-hand_7) // Kinase-like (Kinase-like) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTTGGCTTTTCTTTTGTGGCACTGAGACTTATGTGTGTATTGGTCCAG
Internal bar code:CTTTCTCGATGTTTGCGATCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1571
LEAP-Seq percent confirming:38.4615
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGGCTTGGTTTGGTCTG
Suggested primer 2:TACGGATATGCGCGCTTGAT