| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061887 |
| Chromosome: | chromosome 7 |
| Location: | 3661620 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337505 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTCCCCCGCTAGCTGCGAGCCAGCCTTACTTGCCGCCGCCCCACCAG |
| Internal bar code: | AATCGCTATTGACGCTGGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1446 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCGAGATCATATGGGTCC |
| Suggested primer 2: | GACCTGAAGGCTGTCCTGAC |