Insertion junction: CLIP2.061903_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.061903
Chromosome:chromosome 17
Location:83227
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g696450 (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold 5'UTR
Cre17.g696500 PHC19 Pherophorin-chlamydomonas homolog 19; (1 of 71) PF12499 - Pherophorin (DUF3707) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTCGACGCTCGTCGTGTCCTACGCGACGGGTTCGTGCCACTAAGGGAC
Internal bar code:ACCTTCAGGCTCGTTCTGTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2913
LEAP-Seq percent confirming:77.381
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCATTTGCTTGGTTTCCA
Suggested primer 2:GGACGTGTTTGGCGTAGGTA