Insertion junction: CLIP2.061911_4

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.061911
Chromosome:chromosome 13
Location:1113274
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTGAACCCCACCCAACTCTGGCCGATACAACCACTTGTTCCCATCCCA
Internal bar code:TTCGTTGTGGATGGAGCACCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:201
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACACAGCAGGTACCACATCG
Suggested primer 2:GCAAATGAGCGACTACTGCG