| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061950 |
| Chromosome: | chromosome 4 |
| Location: | 2760403 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223600 | (1 of 1) IPR000104//IPR003072//IPR013328 - Antifreeze protein, type I // Orphan nuclear receptor, NOR1 type // 6-phosphogluconate dehydrogenase, domain 2 | 3'UTR | |
| Cre04.g223650 | FAP30 | (1 of 1) PF02494 - HYR domain (HYR); Flagellar Associated Protein 30 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGGACCCGGCGGGCGGATGGACGCTCTTGCCTTTGCAATCCCAGCG |
| Internal bar code: | ATTGTTCCCTGGGGACTCTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1942 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGCGAGTTGTGGTGTAA |
| Suggested primer 2: | CTTATGCCACATCACACGCG |