| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.061952 |
| Chromosome: | chromosome 8 |
| Location: | 2966993 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374800 | (1 of 1) PTHR16288//PTHR16288:SF0 - WD40 REPEAT PROTEIN 4 // TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT WDR4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTTAAAATGGCTTCGATTAGTTGACGAAACAGGCTGCCGCCGCTTCG |
| Internal bar code: | GTCGTGATTTCGCTTAAGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 960 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCAACGAAGCCAAAACG |
| Suggested primer 2: | CTCATCACACCACCACCCTC |