| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.061955 |
| Chromosome: | chromosome 6 |
| Location: | 7170238 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTCTGTACCCCTCGCCCTTTTCGCTCCCATTCCGTGAACATCGCTCAG |
| Internal bar code: | GCCGTTGCCCTCCTACGGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2567 |
| LEAP-Seq percent confirming: | 69.4444 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAGAGAGCTACCCGTTA |
| Suggested primer 2: | CGTAGGGTGAGGGGTAGGAA |