| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061962 |
| Chromosome: | chromosome 14 |
| Location: | 1536727 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617900 | RPL35 | (1 of 1) K02918 - large subunit ribosomal protein L35e (RP-L35e, RPL35); Cytosolic 80S ribosomal protein L35 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGAAAACGGTTACATTTACGCCTTCAGGGCGAACTTGCGGCTGGGGAA |
| Internal bar code: | TCGATCAGTCCTTTAGATTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5319 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACCAAGGAGCAGGTGAG |
| Suggested primer 2: | GAACGACACGAATGCGGATG |