Insertion junction: CLIP2.061967_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061967
Chromosome:chromosome 10
Location:753790
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g801106 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGCTCGATCGTACACGCACGTCAACGTCTCAGCCGTTGACCAGCAGCG
Internal bar code:GCTACCCAATCGAATTGACGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:4576
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:CGTACGTAGGATGTGTCGGG
Suggested primer 2:CACAGTGTGCGGTAGGATGT