| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061971 |
| Chromosome: | chromosome 6 |
| Location: | 3194403 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276250 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAGTGAAATGCTTTCTTATCAATATGTCTGAGCCTGTGCGCAAAAAAG |
| Internal bar code: | AGAGGGAACTGAGGAGGCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4434 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCATGTTGAGTTGCAAA |
| Suggested primer 2: | CATCCTACGCCATTTGCACG |