| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061975 |
| Chromosome: | chromosome 2 |
| Location: | 6249390 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387150 | HSF1 | (1 of 1) PTHR10015:SF162 - HEAT STRESS TRANSCRIPTION FACTOR A-1A-RELATED; Heat shock transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACGTATGCCTTCCGGCATTTGGGGGCGGGCGCCACGCACACAGGGCGG |
| Internal bar code: | GGGGAAGTGACGTTATAGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1399 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGCGGGTATGTGTAGTC |
| Suggested primer 2: | CCACGTGGGTTAGGGACTTC |