| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.061981 |
| Chromosome: | chromosome 6 |
| Location: | 8018233 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304800 | (1 of 1) PTHR11216//PTHR11216:SF61 - EH DOMAIN // GH06923P | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAATGGAGGGGAGGGTAACCGATTTTGCCCCATGCTCAGAGCCCTGCT |
| Internal bar code: | GAATATGTGTACAAAACGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2725 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCGAAGAGAACTGGAAC |
| Suggested primer 2: | CTCAAGTGCTTCCCCAGGAG |