| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.061983 |
| Chromosome: | chromosome 17 |
| Location: | 5333141 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738000 | AGG2 | Aggregation 2; (1 of 6) PTHR15907:SF21 - DUF614 FAMILY PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAATCCGGCAATGCACGCGCCACGGACAGTATGAAATTCAAGTTGGGCG |
| Internal bar code: | GAAACGTCCGGGGTTGTGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 973 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCGGGTTAGCGGTCTTT |
| Suggested primer 2: | GACCGTACGACGACGACTAC |