| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061985 |
| Chromosome: | chromosome 13 |
| Location: | 3130674 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAAGATGGGGTGCTCGTGTGCCATGTGTTTGGACCGAGCGGCGGCTC |
| Internal bar code: | TAATATTGAGTCTATTTGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3042 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCATTAACGTCACCAGT |
| Suggested primer 2: | CATGTGTGCAGGTTTGTGGG |