| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061986 |
| Chromosome: | chromosome 6 |
| Location: | 7198320 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298600 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGCGCCCTTCAAGATTACAACGCAAGGCCTTGCAAAATAACCAAGGA |
| Internal bar code: | AGGCTGTCCTTCTCAAAATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5707 |
| LEAP-Seq percent confirming: | 93.578 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGTCACAGTCATCCGCA |
| Suggested primer 2: | CTCCTCCCAGTCGGTGTAGA |