Insertion junction: CLIP2.061994_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.061994
Chromosome:chromosome 9
Location:6457977
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g415200 NOM1 nucleolar MIF4G and MA3 domains-containing protein; (1 of 1) K17583 - nucleolar MIF4G domain-containing protein 1 (NOM1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGAATGGAAGTAATGCAGCTCGATCATGACACCAGGGAAGGGCCAAAT
Internal bar code:TACATACGCGCTTTTTTAAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1973
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAAGCAGGGCTCCTATCT
Suggested primer 2:GCATGATGCAGCATGGACTG