Insertion junction: CLIP2.062002_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.062002
Chromosome:chromosome 14
Location:3382819
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre14.g629880 (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACACACACACACACACACACACACACACACACACACACACACAC
Internal bar code:GGGGCCTTTCACGTGACAGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1069
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTGTGTGTGTGTGTG
Suggested primer 2:AGACGAAGAAGACGGCGAAG