| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062006 |
| Chromosome: | chromosome 3 |
| Location: | 2846972 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162250 | RAD1,CGL35,RAD17 | Conserved in the Green Lineage; (1 of 1) K02830 - cell cycle checkpoint protein (HRAD1, RAD17) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCGAGCGGTCCTCGGCTTTGCGAACGTGCGCAGCTTTGCGGCCGCGC |
| Internal bar code: | TTGATCTTGGTGGGGTGTTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCTACGAATTCCGACCG |
| Suggested primer 2: | GTTGTCTCTGGTCGTGTCGT |