Insertion junction: CLIP2.062013_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.062013
Chromosome:chromosome 11
Location:1415768
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre11.g469750 (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACCCACACCCACCCACCCACCCACCCACCCACACACGCACACGCACA
Internal bar code:GGGGATGTTAAAGTGTAAGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1685
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTGCTTGCTGAACACCC
Suggested primer 2:ACAGTCGGTCTAGCCAGCTA