| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.062030 |
| Chromosome: | chromosome 16 |
| Location: | 4554001 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671350 | ARS2 | (1 of 2) IPR012083 - Arylsulfatase; Periplasmic arylsulfatase 2 | 5'UTR |
| lncRNA_TCONS_00091459 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGTGCAGTACAAGGGCATTTTCCAGTGGATGCTGTCTCACGCTTTAC |
| Internal bar code: | TTATTAAAGATTCCGGAAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 93.1034 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCCTACGGGAGCAAGGA |
| Suggested primer 2: | AGCAATATGGCCGCCTTTCT |