Insertion junction: CLIP2.062049_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.062049
Chromosome:chromosome 13
Location:1498334
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g572600 (1 of 5) IPR001214//IPR003616 - SET domain // Post-SET domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTCCCCGTATCAAAGAACCCACACAATCGTCCGCGGGGAAAGAAGAAG
Internal bar code:AGATATCTTAAAACATGATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4906
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:114
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:114

Suggested primers for confirmation by PCR

Suggested primer 1:GCACCAAACCGCTTCATGTT
Suggested primer 2:GAATCATTCGCTGTGTCCGC