| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.062057 |
| Chromosome: | chromosome 6 |
| Location: | 2642461 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270250 | CDC45,DIV65 | (1 of 1) K06628 - cell division control protein 45 (CDC45); Cell Division Cycle protein 45 | 3'UTR |
| Cre06.g800644 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCATATGGTACCTCGAGCCACGCTAGGCCAGTCCGTCTATATGTCTA |
| Internal bar code: | CTACCTCGTAGATTTTGCCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2902 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGTTCCCTCTTCCTGCG |
| Suggested primer 2: | TGTGTGCTGGGTCTTGGTTT |