Insertion junction: CLIP2.062058_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062058
Chromosome:chromosome 1
Location:337483
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g001950 Predicted protein with 3-phosphoshikimate1-carboxyvinyltransferase core domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAACAAGTGCGAGCCGTGACTACATTGCTTAATACGGCTTGGACGAACC
Internal bar code:TCCGCGAGAACGAGAAGTGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1179
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTGCCTCACAAGTTGCC
Suggested primer 2:AACAAGAACACAGCAACGGC