| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.062088 |
| Chromosome: | chromosome 10 |
| Location: | 153871 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCAATTAACAATCCAGTTCCAGGAGACGAGGGGCCCTTGCGGCTTGG |
| Internal bar code: | ACCATGTAGGAGTGGTGAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5286 |
| LEAP-Seq percent confirming: | 85.4545 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGGCAACTTAGCGAGTTT |
| Suggested primer 2: | AATGGAGTTGAGGGCCACTG |