| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.062098 |
| Chromosome: | chromosome 6 |
| Location: | 8344400 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307450 | SPL1 | (1 of 1) K12825 - splicing factor 3A subunit 1 (SF3A1, SAP114); SF3A1 splicing factor 3a, subunit 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCTGACGTGCAGCGTCACCTGCTCTACCTGTACCACGTGGTGACTTG |
| Internal bar code: | GCCTCTGGTAGTCTCTCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3514 |
| LEAP-Seq percent confirming: | 78.125 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATTGTGCGCTGTGTGGGA |
| Suggested primer 2: | AACGAGAAGCTCATTGGCCA |