| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062100 |
| Chromosome: | chromosome 8 |
| Location: | 2850934 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374000 | (1 of 1) PF10444 - Nbl1 / Borealin N terminal (Nbl1_Borealin_N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGCCCTGACAGGCACGCACGCCGCCTCCATTCAAGCACCCAGCCCGC |
| Internal bar code: | CACACGGCGTTGTTCGCCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCGTATGAGGCACAGCG |
| Suggested primer 2: | CTACCACAAACCCCTCCACC |