Insertion junction: CLIP2.062118_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.062118
Chromosome:chromosome 3
Location:558141
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145247 TEF11 S-isoprenylcysteine O-methyltransferase; (1 of 3) 2.1.1.100 - Protein-S-isoprenylcysteine O-methyltransferase / Prenylcysteine carboxyl methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGCAGTGGACAGGAGCGGAGCGCACCACAAACACAGGAGCAGCGCCCC
Internal bar code:TCTTCCTCCATAAAGAACTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:957
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGGTGGGTCCCAATTGT
Suggested primer 2:TCCAGGATGAATCGGACCCT