| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062121 |
| Chromosome: | chromosome 2 |
| Location: | 7479667 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392505 | (1 of 4) PTHR22849//PTHR22849:SF39 - WDSAM1 PROTEIN // SUBFAMILY NOT NAMED | 3'UTR | |
| Cre09.g392542 | (1 of 1) K11423 - histone-lysine N-methyltransferase SETD2 (SETD2, SET2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACACGAACCAAAATTATGCTTGCGTCCCTTCCATACGCGCAAGCCAG |
| Internal bar code: | TTGGCGGATAAAATCGCAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1852 |
| LEAP-Seq percent confirming: | 92.1875 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCACAGTATTGGAGGGC |
| Suggested primer 2: | CTACGAGTAGGCTCTGCAGC |