Insertion junction: CLIP2.062139_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062139
Chromosome:chromosome 8
Location:4046599
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g382750 (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCGAAAGAATTGTCCCCCCCCCCGGATACCCCTCTCACACAATATAAC
Internal bar code:GCCATCTTACAATACCAGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3753
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAGTATGGGCACGTGTC
Suggested primer 2:CTCGCGCTCATGTTTGCTAC