| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062151 |
| Chromosome: | chromosome 2 |
| Location: | 6821164 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388282 | bS18m,MRPS18 | Mitochondrial ribosomal protein S18; (1 of 2) PF01084 - Ribosomal protein S18 (Ribosomal_S18) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGTAAGAACGTGTCCAACCTCCAACCTGCTCCAACCCACACGGACAC |
| Internal bar code: | TCAAGGGGGCGGGATGAGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 156 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTTTGGCACGTTAGCG |
| Suggested primer 2: | AGTGTGCGTGCTCACCAATA |