Insertion junction: CLIP2.062158_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.062158
Chromosome:chromosome 10
Location:6470104
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g465000 (1 of 8) 2.7.12.2 - Mitogen-activated protein kinase kinase / MKK 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTATTCTGGCGCAGACCCAACCGGTTTCCTACTGGCGTAAACCCCAAC
Internal bar code:TATCTAACGGTACTATGTATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2686
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGTGTGTGGCATTGTGG
Suggested primer 2:CACAGCACAAACACGCATCA