| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.062165 |
| Chromosome: | chromosome 3 |
| Location: | 3144220 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164800 | (1 of 1) PTHR24361:SF363 - PROTEIN KINASE-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCAAATAGTCAAGGGATCCCGCCTCTGGCTGAGCAACCGCCAGCATG |
| Internal bar code: | AGGGCCTAGTAGCGTGAAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2180 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCCCTTTCGCTCTTTCAT |
| Suggested primer 2: | TTCGAGATTGTGTGCCGGAA |