Insertion junction: CLIP2.062177_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.062177
Chromosome:chromosome 5
Location:1076218
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre05.g247850 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGGGGGTTGAGGTTTCAGGGATGAAGTTCAACTGTGTGCGGCTAACTC
Internal bar code:CTACATCACAACATCTGTTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:392
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAGGCAGCGGCAAGAAAA
Suggested primer 2:CTGATGCTGATGGTGGTGGT