| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.062181 |
| Chromosome: | chromosome 1 |
| Location: | 2078338 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011550 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACGCGACAGGCCAGGTACTAGGCGTTCGCCTTGCCTAAGCCGCAAAA |
| Internal bar code: | AAGGATGTTTGGGTACAACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2245 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTCCGACTCTTTGCAGT |
| Suggested primer 2: | GATCCCGTCGTCCTCGTAAC |