| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.062182 |
| Chromosome: | chromosome 12 |
| Location: | 3304273 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498900 | RPS7 | (1 of 1) K02993 - small subunit ribosomal protein S7e (RP-S7e, RPS7); Cytosolic 80S ribosomal protein S7 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCGGGCACGCAGGCGCCCAAGCTTACAACATCTCAGACGGTTTCATA |
| Internal bar code: | AGTCGAGTTGATAATGAAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5495 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCAGTGAAATGCTCGAT |
| Suggested primer 2: | GAACAGGGCCTGCAAAAGTG |