| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062190 |
| Chromosome: | chromosome 6 |
| Location: | 5954797 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g290100 | SYP6 | Early endosomal Qc-SNARE protein, Syx6/Tlg1/Syntaxin 6-family (Qc.III.b); (1 of 1) K08498 - syntaxin 6 (STX6) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAGAACGACTAGTCGCGCTGCATGAGTCTAATTCTACATTCCCGTGC |
| Internal bar code: | TTAATGGCTGCTGACTAATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1156 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAAGATAGCCGTCCTCC |
| Suggested primer 2: | ACACATGCGAGGTTGAGAGG |