| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062198 |
| Chromosome: | chromosome 5 |
| Location: | 712872 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245350 | (1 of 1) IPR003582//IPR009011 - ShKT domain // Mannose-6-phosphate receptor binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCAGCACTGGCCAAGTCAAGAGAAGCAGCACGCATTGCCCGCGCAT |
| Internal bar code: | CAGACCGCTAGTGAGCATTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1326 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGGCGGGCTTTCTTTTA |
| Suggested primer 2: | AACCCAGCCCCATACATGTG |