| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062207 |
| Chromosome: | chromosome 6 |
| Location: | 5516671 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286600 | (1 of 1) PF13898 - Domain of unknown function (DUF4205) (DUF4205) | 3'UTR | |
| Cre06.g286650 | DMT4,DMC4 | (1 of 4) K00558 - DNA (cytosine-5)-methyltransferase 1 (DNMT1, dcm); Putative DNA methylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTATGTGTGAATGAGCCTCGACTGCAACCAACTGTTGGATGCCCGGC |
| Internal bar code: | TGCCTCGTCTGGGCGCAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1601 |
| LEAP-Seq percent confirming: | 92.5926 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCCTTGGTTTGCCCAGT |
| Suggested primer 2: | GGCATGACCCCATTCTGTGA |