| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.062216 |
| Chromosome: | chromosome 8 |
| Location: | 2016128 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g368650 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTGCCAAAACGGTCAGCGGGTCAACCCAACCACCTCCGCGTTGACT |
| Internal bar code: | GCCGCCGGATAGACTATATGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2119 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGCAGTTCACAAGCAC |
| Suggested primer 2: | CAACGAAAGCACCCACCTTG |