| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062223 |
| Chromosome: | chromosome 12 |
| Location: | 8857894 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546400 | DLR2,DLC7b,DCL7B,LC7B | (1 of 2) K10419 - dynein light chain roadblock-type (DYNLRB, DNCL2); Roadblock/LC7 Family Light Chain | 5'UTR |
| Cre12.g546450 | (1 of 1) PTHR12472//PTHR12472:SF0 - RAB3-GAP REGULATORY DOMAIN // RAB3 GTPASE-ACTIVATING PROTEIN NON-CATALYTIC SUBUNIT | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGACTACCTAACGCAAATAAGACGTTCACTGCAAGCCAGACCTACCA |
| Internal bar code: | AGTATAACGAGTCTGTTACGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2145 |
| LEAP-Seq percent confirming: | 18.75 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTTCCCTCGTGACAGAA |
| Suggested primer 2: | CTGCTTGTATTGATGGCGCC |