Insertion junction: CLIP2.062246_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062246
Chromosome:chromosome 6
Location:4153504
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278231 (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACTCATGTCCGAACGTTAGAAGCGTGAAATATTGCCATGATGATAGCA
Internal bar code:CTCTGACTTGATCGCAGGATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3555
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTGTTGTCGTGTCTGGAG
Suggested primer 2:TTAACACACGCACGTTGCTG