| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062247 |
| Chromosome: | chromosome 9 |
| Location: | 55773 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g800964 | (1 of 31) PF03732 - Retrotransposon gag protein (Retrotrans_gag) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTTGAGATAGAACCCAACCGGAGAGACGATAGAGGTGGAGGAGAAAG |
| Internal bar code: | GTACTGCGGGGAAGTGGACATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2880 |
| LEAP-Seq percent confirming: | 47.8261 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCCCTCTCTCTCCAGCC |
| Suggested primer 2: | ACATGTAAGTGGGGTCGCAG |