Insertion junction: CLIP2.062248_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.062248
Chromosome:chromosome 12
Location:8863110
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g546300 (1 of 1) PF09728 - Myosin-like coiled-coil protein (Taxilin) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCAAGGTGGGCGACACCACGACGCCATGCTAGCTAGCAATCTATTCG
Internal bar code:TCTAGCCCTTGTAGTGCCGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:3602
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAGACCATCGTCAAGCTG
Suggested primer 2:GTCGTTTTCAACCCAGCCAC