| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.062249 |
| Chromosome: | chromosome 6 |
| Location: | 317930 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250950 | (1 of 1) K12836 - splicing factor U2AF 35 kDa subunit (U2AF1); U2 snRNP auxiliary factor, small subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGGTCCTAGCGCAGTTGGCACACTTGAGACCTCCTCACAATGCGAGC |
| Internal bar code: | CCCGGCTGTTCTGGTAGGAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2780 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTAGAGCTCACCTCGAAG |
| Suggested primer 2: | CCATGGTCCTGTCTCACCAC |